CURRICULUM VITAE
Go Suzuki
ADDRESS
4-698-1 Asahigaoka, Kashiwara, Osaka 582-8582, Japan
Office phone: +81 729 78 3660
FAX No.: +81 729 78 3660
e-mail : gsuzuki@cc.osaka-kyoiku.ac.jp
SEX
Male
MARITAL STATUS
Married
DATE OF BIRTH
11-October-1971
CITIZENSHIP
Japanese
CURRENT POSITION
Ph. D.
Professor
Laboratory of Plant Molecular Genetics, Division of Natural Science, Osaka Kyoiku University
ACADEMIC QUALIFICATION
1994, B. Sc. (Agriculture), Lab. of Plant Breeding, Tohoku University.
1996, M. Sc. (Agriculture), Lab. of Plant Breeding, Tohoku University.
1999, Ph. D. (Agriculture), Lab. of Plant Breeding, Tohoku University.
.........Research Fellow of the Japan Society for the Promotion of Science.
Thesis title: Genomic organization of the S locus and the S-multigene family in self-incompatible Brassica campestris.
RESEARCH PROFILE AND OUTSTANDING ACHIEVEMENTS
------Molecular genetics of self-incompatibility in Brassica.
1. Genomic organization of the S locus.
. I have cloned two self-incompatibility genes, SLG9 and SRK9 from an S9 homozygote of B. campestris. The two linked genes, SLG9 and SRK9, were quite similar in the long 5'-flanking regions as well as in the coding regions corresponding to the S domain, indicating that duplication or conversion may be a one of the mechanisms to produce a new set of SLG and SRK. This result has been published in "Gene" in 1997.
. I separately introduced the SLG9 and SRK9 into tobacco plants. The presence of an SLG9-like transcript in SRK9 transformants indicated the possibility of alternative polyadenylation sites in the SRK9 gene. This result has been published in "Plant and Cell Physiology" in 1996. Function of the SLG and SRK genes in the self-incompatibility recognition in stigmas was confirmed by further experiments (Takasaki et al. 2000).
. I successfully cloned the MluI-digested 76-kb fragment, containing both SLG9 and SRK9 genes, into a P1 derived artificial chromosome (PAC) vector. It is the first report of using an artificial chromosome-based vector to clone a region of the S locus. This direct cloning method is useful in analyzing the complex genomic regions. This result has been published in "Gene" in 1997.
. I dissected the 76-kb S-locus, and found 14 expressed genes in this regions. One of the genes, named SP11, encoded a small cysteine-rich protein, and was expressed specifically in anthers. SP11 was similar to a pollen coat protein, PCP-A1, which had previously been shown to bind SLG, suggesting that SP11 was a candidate of the pollen S gene. This result has been published in "Genetics" in 1999; this is the first report of the cloning of the pollen S gene in Brassica. Function of the SP11 gene in the self-incompatibility recognition in pollen was confirmed by further several experiments (Takayama et al. 2000, Watanabe et al. 2000, Shiba et al. 2001).
. I performed PFGE blot analysis to determine physical distances between the S locus genes in several S haplotypes of B. campestris and B. oleracea. The tendency for a shoter physical distance between SLG and SRK in B. campestris than B. oleracea was observed. This result has been published in "Theoretical and Applied Genetics" in 2001.
2. Molecular analysis of the S-multigene family.
. I have cloned members of the S-multigene family from an S9 homozygote of B. campestris, showing that SLG, SRK and S-related genes form a large S-multigene family in B. campestris. This result has been published in "Plant and Cell Physiology" in 1995. Furthermore, three members of the S-multigene family are clustered and linked to the S locus. The physical distance between the regions of the SLG and SRK genes and the three homologs was estimated as within 610 kb by PFGE analysis. This result has been published in "Molecular and General Genetics" in 1997. Sequence comparison and linkage analyhsis of all members of the S-multigene family were performed by further experiments (Kai et al. 2001).
------Genomic analysis in onion.
1. Constsruction of an onion BAC library.
. I constructed a bacterial artificial chromosome (BAC) libray of onion (Allium cepa). Average insert size of the library is 100 kb. A total of 48,000 clones, that correspond to 0.32 genome equivalent, were stocked in 480 glycerol stocks (corresponding to five 96-well plates), each of which contains 100 independent clones. I can screen genes of interest by PCR screening from 3D superpools. This result has been published in "Breeding Science" in 2002.
2. BAC FISH experiment in onion.
. Fluorescent in situ hybridization (FISH) screening resulted in finding the BAC clones localized on centromeric, telomeric, or several restricted chromosomal regions, although most of the clones hybridized with entire chromosomes. One of the clones showing the restricted chromosomal regions could contain 18S-5.8S-26S rDNA related sequences. The BAC FISH screening is a powerful method for identification of the molecular cytogenetic markers in large genome plants. This result has been published in "Genes and Genetic Systems" in 2001.
------Characterization of wheat centromeres.
. Cereal centromeres commonly contain many repetitive sequences that are derived from Ty3/gypsy retrotransposon. FISH analysis using a large DNA insert library of wheat identified a 67-kb clone that showed strong hybridization signals on the centromeres. The clone contains Ty3/gypsy retrotransposon-related sequences; both integrase and CCS1 family sequences were identified. Subsequently, additional 23 large-insert clones, which also contained the integrase and CCS1 sequences, were isolated. Based on the number of the integrase repeats in the clones determined by DNA gel blot analysis, I concluded that the retrotransposon-like sequences are tandemly repeated in wheat centromeres in ca. 55-kb interval on average. This conclusion is consistent with the results of FISH analysis on the extended DNA fibers. This result has been published in "Plant and Cell Physiology" in 2001.
------Genomic organization of SBEI locus in wheat.
. A wheat SBEI (Starch Branching Enzyme I) multigene family is related for the synthesis of starch. In collaboration with Dr. S. Rahman (CSIRO, Australia), I characterized the 120-kb and 100-kb clones containing SBEI genes from a large-inserts genomic library of Ae. Squarrosa. DNA gel blot analyses showed that both the 120-kb and 100-kb clones contained the same overlapped region, including SBEI-D2, -D3, and -D4. Genomic DNA gel blot analysis, PFGE analysis and Fiber FISH analysis showed that at least three SBEI-related sequences (D2,D3 and D4) were located in tandem, and another sequence similar to SBEI was possibly located near the cluster. This result has been published in "Functional & Integrative Genomics" in 2003.
TECHNICAL EXPERTISE
* Construction of a genomic library and a cDNA library.
* Isolation and purification of total DNA, total RNA, mRNA from plant tissues.
* Transformation of rice and tobacco plants.
* Molecular analysis with non-RI method(Southern, Northern, Western blot analysis, RT-PCR analysis, RFLP mapping, physical mapping, sequencing etc.).
* Techniques for high molecular weight DNA (isolation, electrophoresis, gel extraction and cloning of high molecular weight DNA).
* Bud-pollination of Brassica for obtaining the F1 and F2 progeny.
* Pollination assay and PCR-RFLP for determination of self-incompatibility phenotype.
LIST OF PRESENTATION
----Publications in international refereed journals.
Fujimoto, T., Okumura, `., Yoshido, A., Yasukochi, Y., Suzuki, G., and Sahara, K. (2018) Construction of a BAC library and selection of BACs containing orthologs of Bombyx mori genes in Stenopsyche marmorata (Trichoptera: Stenopsychidae). J. Insect Biotech. Sericol. 87: 61-69.
Takada, Y., Murase, K., Shimosato-Asano, H., Sato, T., Nakanishi, H., Suwabe, K., Shimizu, K.K., Lim, Y.P., Takayama, S., Suzuki, G., and Watanabe, M. (2017) Duplicated pollen-pistil recognition loci control intraspecific unilateral incompatibility in Brassica rapa. Nature Plants 3:17096.
Yasuda, S., Wada, Y., Kakizaki, T., Tarutani, Y., Miura-Uno, E., Murase, K., Fujii, S., Hioki, T., Shimoda, T., Takada, Y., Shiba, H., Takasaki-Yasuda, T., Suzuki, G., Watanabe, M., and Takayama, S. (2016) Complex dominance hierarchy controlled by polymorphism of small RNAs and their targets. Nature Plants 3:16206.
Maeda, S., Sakazono, S., Masuko-Suzuki, H., Taguchi, M., Yamamura, K., Nagano, K., Endo, T., Saeki, K., Osaka, M., Nabemoto, M., Ito, K., Kudo, T., Kobayashi, M., Kawagishi, M., Fujita, K., Nanjo, H., Shindo, T., Yano, K., Suzuki, G., Suwabe, K., and Watanabe, M. (2016) Comparative analysis of microRNA profiles of rice anthers between cool-sensitive and cool-tolerant cultivars under cool-temperature stress. Genes Genet. Syst. 91: 97-109.
Kudo, T., Sasaki, Y., Terashima, S., Matsuda-Imai, N., Takano, T., Saito, M., Kanno, M., Ozaki, S., Suwabe, K., Suzuki, G., Watanabe, M., Matsuoka, M., Takayama, S., and Yano, K. (2016) Identification of reference genes for quantitative expression analysis using large-scale RNA-seq data of Arabidopsis thaliana and model crop plants. Genes Genet. Syst. 91: 111-125.
Rabiger, D.S., Taylor, J.M., Spriggs, A., Hand, M.L., Henderson, S.T., Johnson, S.D., Oelkers, K., Hrmova, M., Saito, K., Suzuki, G., Mukai, Y., Carroll, B.J., and Koltunow, A.M.G (2016) Generation of an integrated Hieracium genomic and transcriptomic resource enables exploration of small RNA pathways during apomixis initiation. BMC Biology 14: 86.
Matsuba, A., Fujii, M., Lee, S.S., Suzuki, G., Yamamoto, M. and Mukai, Y. (2015) Molecular cytogenetic use of BAC clones in Neofinetia falcata and Rhynchostylis coelestis. The Nucleus 58: 207-210.
Mukai, Y., Okamoto, G., Kiryu, S., Takemoto, S., Sharma, S.K., Suzuki, G. and Yamamoto, M. (2015) The D-genome plays a critical role in the formation of haploid Aegilops tauschii through Imperata cylindrica mediated uniparental chromosome elimination. The Nucleus 58: 199-206.
Matsuda, T., Matsushima, M., Nabemoto, M., Osaka, M., Sakazono, S., Masuko-Suzuki, H., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Okumura, K., Suzuki, G., Watanabe, M., and Suwabe, K. (2015) Transcriptional characteristics and differences in Arabidopsis stigmatic papilla cells pre- and post-pollination. Plant Cell Physiol. 56: 663-673.
Ohyanagi, H., Takano, T., Terashima, S., Kobayashi, M., Kanno, M., Morimoto, K., Kanegae, H., Sasaki, Y., Saito, M., Asano, S., Ozaki, S., Kudo, T., Yokoyama, K., Aya, K., Suwabe, K., Suzuki, G., Aoki, K., Kubo, Y., Watanabe, M., Matsuoka, M., and Yano, K. (2015) Plant Omics Data Center: an integrated web repository for interspecies gene expression networks with NLP-based curation. Plant Cell Physiol. 56: e9.
Fujiwara, M., Suzuki, G., Kudo, D., Oba, H., Wada, Y., Wada, H., Wada, N., Rahman, S., Fukui, K., and Mukai, Y. (2014) Localization of transgene-derived friabilins in rice endosperm cells. Plant Biotech. 31: 67-70.
Kotani, Y., Henderson, S.T., Suzuki, G., Johnson, S.D., Okada, T., Siddons, H., Mukai, Y., and Koltunow, A.M.G. (2014) The LOSS OF APOMEIOSIS (LOA) locus in Hieracium praealtum can function independently of the associated large-scale repetitive chromosomal structure. New Phytologist 201: 973-981.
Sudo, K., Park, J.-I, Sakazono, S., Masuko-Suzuki, H., Osaka, M., Kawagishi, M., Fujita, K., Maruoka, M., Nanjo, H., Suzuki, G., Suwabe, K., and Watanabe, M. (2013) Demonstration in vivo of the role of Arabidopsis PLIM2 actin-binding proteins during pollination. Genes Genet. Syst. 88: 279-287.
Osaka, M., Matsuda, T., Sakazono, S., Masuko-Suzuki, H., Maeda, S., Sewaki, M., Sone, M., Takahashi, H., Nakazono, M., Iwano, M., Takayama, S., Shimizu, K.K., Yano, K., Lim, Y.P., Suzuki, G., Suwabe, K., and Watanabe, M. (2013) Cell type-specific transcriptome of Brassicaceae stigmatic papilla cells from a combination of laser microdissection and RNA sequencing. Plant Cell Physiol. 54: 1894-1906.
Hiroi, K., Sone, M., Sakazono, S., Osaka, M., Masuko-Suzuki, H., Matsuda, T., Suzuki, G., Suwabe, K., and Watanabe, M. (2013) Time-lapse imaging of self- and cross-pollinations in Brassica rapa. Ann. Bot. 112: 115-122.
Takada, Y., Sato, T., Suzuki, G., Shiba, H., Takayama, S., and Watanabe, M. (2013) Involvement of MLPK pathway in intraspecies unilateral incompatibility regulated by a single locus with stigma and pollen factors. Genes Genomes Genetics (G3) 3: 719-726.
Suzuki, G., Nishiuchi, C., Tsuru, A., Kako, E., Li, J., Yamamoto, M., and Mukai, Y. (2013) Cellular localization of mitotic RAD21 with repetitive amino acid motifs in Allium cepa. Gene 514: 75-81.
Watanabe, M., Suwabe, K., and Suzuki, G. (2012) Molecular genetics, physiology and biology of self-incompatibility in Brassicaceae. Proc. Jpn. Acad., Ser. B 88: 519-535.
Suwabe, K., Suzuki, G., Nunome, T., Hatakeyama, K., Mukai, Y., Fukuoka, H., and Matsumoto, S. (2012) Microstructure of a Brassica rapa genome segment homoeologous to the resistance gene cluster on Arabidopsis chromosome 4. Breeding Sci. 62: 170-177.
Masamura, N., McCallum, J., Khrustaleva, L., Kenel, F., Pither-Joyce, M., Shono, J., Suzuki,G., Mukai, Y., Yamauchi, N., and Shigyo, M. (2012) Chromosomal organization and sequence diversity of genes encoding lachrymatory factor synthase in Allium cepa L. Genes Genomes Genetics (G3) 2: 643-651.
Suzuki,G., Ogaki, Y., Hokimoto, N., Xiao, L., Kikuchi-Taura, A., Harada, C., Okayama, R., Tsuru, A., Onishi, M., Saito, N., Do, G.S., Lee, S.H., Ito, T., Kanno, A., Yamamoto, M., and Mukai, Y. (2012) Random BAC FISH of monocot plants reveals differential distribution of repetitive DNA elements in small and large chromosome species. Plant Cell Rep. 31: 621-628.
Aya, K., Suzuki, G., Suwabe, K., Hobo, T., Takahashi, H., Shiono, K., Tsutsumi, N., Nakazono, M., Nagamura, Y., Matsuoka, M., and Watanabe, M. (2011) Comprehensive network analysis of anther-expressed genes in rice by the combination of 33 laser microdissection and 143 spatiotemporal microarrays. PLoS ONE 6: e26162.
Okada, T., Ito, K., Johnson, S.D., Oelkers, K., Suzuki, G., Houben, A., Mukai, Y., and Koltunow, A.M.G. (2011) Chromosomes carrying meiotic avoidance loci in three apomictic eudicot Hieracium subgenus Pilosella species share structural features with two monocot apomicts. Plant Physiol. 157: 1327-1341.
Suzuki, G., Wada, H., Goto, H., Nakano, A., Oba, H., Deno, T., Rahman, S., and Mukai, Y. (2011) Transgenic rice plants harboring the grain hardness-locus region of Aegilops tauschii. Plant Cell Rep. 30: 2293-2301.
Nagaki, K., Shibata, F., Suzuki, G., Kanatani, A., Ozaki, S., Hironaka, A., Kashihara, K., and Murata, M. (2011) Coexistence of NtCENH3 and two retrotransposons in tobacco centromeres. Chromosome Res. 19: 591-605.
Koltunow, A.M.G., Johnson, S.D., Rodrigues, J.C.M., Okada, T., Hu, Y., Tsuchiya, T., Wilson, S., Fletcher, P., Ito, K., Suzuki, G., Mukai, Y., Fehrer, J. and Bicknell, R.A. (2011) Sexual reproduction is the default mode in apomictic Hieracium subgenus Pilosella where two dominant loci function to enable apomixis. Plant J. 66: 890-902.
Suzuki, G., Shiomi, M., Morihana, S., and Mukai, Y. (2010) Characterization of CDC48 in Allium cepa. Chromosome Sci. 13: 27-29.
Suwabe, K, Suzuki, G., and Watanabe, M. (2010) Achievement of Genetics in Plant Reproduction Research: the past decade for the coming decade. Genes Genet. Syst. 85: 297-310.
Suzuki, G., Shiomi, M., Morihana, S., Yamamoto, M., and Mukai, Y. (2010) DNA methylation and histone modification in onion chromosomes. Genes Genet. Syst. 85: 377-382.
Tarutani, Y., Shiba, H., Iwano, M., Kakizaki, T., Suzuki, G., Watanabe, M., Isogai, A., and Takayama, S. (2010) Trans-acting small RNA determines dominance relationships in Brassica self-incompatibility. Nature 466: 983-986.
Oda. S., Kaneko, F, Yano, K, Fujioka, T., Masuko, H., Park, J.-I., Kikuchi, S., Hamada, K., Endo, M., Nagano, K., Nagamura, Y., Kawagishi-Kobayashi, M., Suwabe, K, Suzuki, G., and Watanabe, M. (2010) Morphological and gene expression analysis under cool temperature conditions in rice anther development. Genes Genet. Syst. 85: 107-120.
Park, J.-I., Ishimizu, T., Suwabe, K., Sudo, K., Masuko, H., Hakozaki, H., Nou, I.-S., Suzuki, G., and Watanabe, M. (2010) UDP-glucose pyrophosphorylase is rate limiting in vegetative and reproductive phases in Arabidopsis thaliana. Plant Cell Physiol. 51: 981-996.
Isokawa, S., Osaka, M., Shirasawa, A., Kikuta, R., Komatsu, S., Horisaki, A., Niikura, S., Takada, Y., Shiba, H., Isogai, A., Takayama, S., Suzuki, G., Suwabe, K., and Watanabe, M. (2010) Novel self-compatible lines of Brassica rapa L. isolated from the Japanese bulk-populations. Genes Genet. Syst. 85: 87-96.
Tsuchimatsu, T., Suwabe, K., Shimizu-Inatsugi, R., Isokawa, S., Pavlidis, P., Stadler, T., Suzuki, G., Takayama, S., Watanabe, M., and Shimizu, K.K. (2010) Evolution of self-compatibility in Arabidopsis by a mutation in the male specificity gene. Nature 464: 1342-1346.
Wada, N., Kajiyama, S., Cartagena, J.A., Lin, L., Akiyama, Y., Otani, M., Suzuki, G., Mukai, Y., Aoki, N., and Fukui, K. (2010) The effects of puroindoline b on the ultrastructure of endosperm cells and physicochemical properties of transgenic rice plant. J. Cereal Sci. 51: 182-188.
Suzuki, G. (2009) Recent progress in plant reproduction research: the story of the male gametophyte through to successful fertilization. Plant Cell Physiol. 50: 1857-1864.
Imazawa, T., Suzuki, G., Nakano, A., Yamamoto, M., and Mukai, Y. (2009) Visualization of multiple T-DNA loci by FISH on extended DNA fibers. Plant Biotech. 26: 421-425.
Wada, N., Kajiyama, S., Akiyama, Y., Kawakami, S., No, D., Uchiyama, S., Otani, M., Shimada, T., Nose, N., Suzuki, G., Mukai, Y., and Fukui, K. (2009) Bioactive beads-mediated transformation of rice with large DNA fragments containing Aegilops tauschii genes. Plant Cell Rep. 28: 759-768.
Watanabe, M., Suzuki, G. and Takayama, S. (2008) Milestones identifying self-incompatibility genes in Brassica species: From old stories to new findings. In: Franklin-Tong VE ed. Self-Incompatibility in Flowering Plants - Evolution, Diversity, and Mechanisms. Berlin, Heidelberg: Springer-Verlag. pp.151-172.
Hobo, T., Suwabe, K., Aya, K., Suzuki, G., Yano, K., Ishimizu, T., Fujita, M., Kikuchi, S., Hamada, K., Miyano, M., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Takahashi, H., Shiono, K., Nakazono, M., Tsutsumi, N., Nagamura, Y., Kurata, N., Watanabe, M., and Matsuoka, M. (2008) Various spatiotemporal expression profiles of anther-expressed genes in rice. Plant Cell Physiol. 49: 1417-1428.
Suwabe, K., Suzuki, G., Takahashi, H., Shiono, K., Endo, M., Yano, K., Fujita, M., Masuko, H., Saito, H., Fujioka, T., Kaneko, F., Kazama, T., Mizuta, Y., Kawagishi-Kobayashi, M., Tsutsumi, N., Kurata, N., Nakazono, M. and Watanabe, M. (2008) Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Plant Cell Physiol. 49: 1407-1416.
Jakse, J., Meyer, J.D.F., Suzuki, G., McCallum, J., Cheung, F., Town, C.D. and Havey, M.J. (2008) Pilot sequencing of onion genomic DNA reveals fragments of transposable elements, low gene densities, and significant gene enrichment after methyl filtration. Mol. Genet. Genomics 280: 287-292.
Fujioka, T., Kaneko, F., Kazama, T., Suwabe, K., Suzuki, G., Makino, A., Mae, T., Endo, M., Kawagishi-Kobayashi, M. and Watanabe, M. (2008) Identification of small RNAs in late developmental stage of rice anthers. Genes Genet. Syst. 83: 281-284.
Hakozaki, H., Park, J.-I., Endo, M., Takada, Y., Kazama, T., Takeda, Y., Suzuki, G., Kawagishi-Kobayashi, M. and Watanabe, M. (2008) Expression and developmental function of the 3-ketoacyl-ACP synthase2 gene in Arabidopsis thaliana. Genes Genet. Syst. 83: 143-152.
Komeda, N., Chaudhary, H., Suzuki, G. and Mukai, Y. (2007) Cytological evidence for chromosome elimination in wheat x Imperata cylindrica hybrids. Genes Genet. Syst. 82: 241-248.
Kakizaki. T., Takada, Y., Fujioka, T., Suzuki, G., Satta, Y., Shiba, H., Isogai, A., Takayama, S. and Watanabe, M. (2006) Comparativeanalysis of the S-intergenic region in class-II S haplotype of self-incompatible Brassica rapa (syn. campestris). Genes Genet. Syst. 81: 63-67.
Ito, T., Suzuki, G., Ochiai, T., Nakada, M., Kameya, T. and Kanno, A. (2005) Genomic organization of the AODEF gene in Asparagus officinalis L. Genes Genet. Syst. 80: 95-103.
Nakano, A., Suzuki, G., Yamamoto, M., Turnbull, K., Rahman, S. and Mukai, Y. (2005) Rearrangements of large-insert T-DNAs in transgenic rice. Mol. Genet. Genomics 273: 123-129.
Takada, Y., Nakanowatari, T., Sato, J., Hatakeyama, K., Kakizaki, T., Ito, A., Suzuki, G., Shiba, H., Takayama, S., Isogai, A. and Watanabe, M. (2005) Genetic analysis of novel intra-species unilateral incompatibility in Brassica rapa (syn. campestris) L. Sex. Plant Reprod. 17: 211-217.
Shiba, H., Park, J.-I., Suzuki, G., Matsushita, M., Nou, I.-S., Isogai, A., Takayama, S. and Watanabe, M. (2004) Duplicated SP11 genes produce alternative transcripts in the S15 haplotype of Brassica oleracea. Genes Genet. Syst. 79: 87-93.
Suzuki, G., Tanaka, S., Yamamoto, M., Tomita, R.N., Kowyama, Y. and Mukai, Y. (2004) Visualization of the S-locus region in Ipomoea trifida: toward positional cloning of self-incompatibility genes. Chromosome Res. 12: 475-481.
Do, G.S., Suzuki, G. and Mukai, Y. (2004) Genomic organization of a novel root alliinase gene, ALL1, in onion. Gene 325: 17-24.
Tomita, R.N., Suzuki, G., Yoshida, K., Yano, Y., Tsuchiya, T., Kakeda, K., Mukai, Y. and Kowyama, Y. (2004) Molecular characterization of a 313-kb genomic region containing the self incompatibility locus of Ipomoea trifida, a diploid relative of sweet potato.@Breeding Sci. 54: 165-175.
Suzuki, G., Kakizaki, T., Takada, Y., Shiba, H., Takayama, S., Isogai, A. and Watanabe, M. (2003) The S haplotypes lacking SLG in the genome of Brassica rapa. Plant Cell Rep. 21: 911-915.
Shiba, H., Kenmochi, M., Sugihara, M., Iwano, M., Kawasaki, S., Suzuki, G., Watanabe, M., Isogai, A. and Takayama, S. (2003) Genomic organization of the S locus region of the Brassica. Biosci. Biotech. Biochem. 67: 622-626.
Kakizaki, T., Takada, Y., Ito, A., Suzuki, G., Shiba, H., Takayama, S., Isogai, A. and Watanabe, M. (2003) Linear dominant-recessive relationship between S alleles in pollen is directed by the expression of SP11 in Brassica self-incompatibility. Plant Cell Physiol. 44: 70-75.
Suzuki, G., Moriyama, M., Fujioka, K., Yamamoto, M., Subramanyam, N.C., Li, Z., Appels, R., Morell, M., Mukai, Y. and Rahman, S. (2003) The starch branching enzyme I locus from Aegilops tauschii, the donor of the D genome to wheat. Funct. Integr. Genomics 3: 69-75.
Suzuki, G., Do, G.S. and Mukai, Y. (2002) Efficient storage and screening system for onion BAC clones. Breeding Sci. 52: 157-159.
Suzuki, G., Ura, A, Saito, N., Do, G.S., Seo, B.B., Yamamoto, M. and Mukai, Y. (2001) BAC FISH analysis in Allium cepa. Genes Genet. Syst. 76: 251-255.
Takada, Y., Ito, A., Ninomiya, C., Kakizaki, T., Takahata, Y., Suzuki, G., Hatakeyama, K., Hinata, K., Shiba, H., Takayama, S. and Isogai, A. and Watanabe, M. (2001) Characterization of expressed genes in the SLL2 region of self-compatible Arabidopsis thaliana. DNA Res. 8: 215-219.
Shiba, H., Takayama, S., Iwano, M., Shimosato, H., Funato, M., Nakagawa, T., Suzuki, G., Watanabe, M., Hinata, K. and Isogai, A. (2001) A pollen coat protein, SP11/SCR, determines the pollen S-specificity in the self-incompatibility of Brassica. Plant Physiol. 125: 2095-2103.
Hatakeyama, K., Takasaki, T., Suzuki, G., Nishio, T., Watanabe, M., Isogai, A. and Hinata, K. (2001) The S receptor kinase gene determines dominance relationships in stigma expression of self-incompatibility in Brassica. Plant J. 26: 69-76.
Kai, N., Suzuki, G., Watanabe, M., Isogai, A. and Hinata, K. (2001) Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome. Mol. Genet. Genomics 265: 526-534.
Do, G.S., Seo, B.B., Oh, S.J., Yamamoto, M., Suzuki, G. and Mukai, Y. (2001) Identification and chromosomal location of tandemly repeated DNA sequences in Allium cepa. Genes Genet. Syst. 76: 53-60.
Fukui, K.-N., Suzuki, G., Lagudah, E.S., Rahman, S., Appels, R., Yamamoto, M. and Mukai, Y. (2001) Physical arrangement of retrotransposon-related repeats in centromeric regions of wheat. . Plant Cell Physiol. 42: 189-196.
Suzuki, G., Watanabe, M. and Nishio, T. (2000) Physical distances between S-locus genes in various S haplotypes of Brassica rapa and B. oleracea. Theor. Appl. Genet. 101: 80-85.
Watanabe, M., Ito, A., Takada, Y., Ninomiya, C., Kakizaki, T., Takahata, Y., Hatakeyama, K., Hinata, K., Suzuki, G., Takasaki, T., Satta, S., Shiba, H., Takayama, S. and Isogai, A. (2000) Highly diverged sequences of the pollen self-incompatibility (S) gene in the class-I S haplotypes of Brassica campestris (syn. rapa) L. FEBS lett. 473: 139-144.
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Watanabe, M., Suzuki, G., Takayama, S., Isogai, A. and Hinata, K. (2000) Genomic organization of the SLG/SRK region of the S locus in Brassica species. Ann. Bot. 85A: 155-160.
Suzuki, G., Kai, N., Hirose, T., Fukui, K., Nishio, T., Takayama, S., Isogai, A., Watanabe, M. and Hinata, K. (1999) Genomic organization of the S locus: Identification and characterization of genes in SLG/SRK region of S9 haplotype of Brassica campestris (syn. rapa). Genetics 153: 391-400.
Watanabe, M., Suzuki, G., Toriyama, K., Takayama, S., Isogai, A. and Hinata, K. (1999) Two anther-expressed genes on downstream of SLG9: identification of a novel S-linked gene specifically expressed in anthers at the uninucleate stage of Brassica campestris (syn. rapa) L. Sex. Plant Reprod. 12: 127-134.
Watanabe, M., Suzuki, G., Hatakeyama, K., Toriyama, K., Isogai, A. and Hinata, K. (1999) Molecular biology of self-incompatibility in Brassica species. Plant Biotech. 16: 263-272.
Iwano, M., Sakamoto, K., Suzuki, G., Watanabe, M., Takayama, S., Fukui, K., Hinata, K. and Isogai, A. (1998) Visualization of a self-incompatibility gene in Brassica campestris L. by multicolor FISH. Theor. Appl. Genet. 96: 751-757.
Sasaki, Y., Iwano, M., Matsuda, N., Suzuki, G., Watanabe, M., Isogai, A. and Toriyama, K. (1998) Localization of an SLG protein expressed under the regulation of a tapetum-specific promoter in anthers of transgenic Brassica napus. Sex. Plant Reprod. 11: 245-250.
Watanabe, M., Watanabe, M., Suzuki, G., Shiba, H., Takayama, S., Isogai, A. and Hinata, K. (1998) Sequence comparison of four SLR1 alleles in Brassica campestris (syn. B. rapa). Sex. Plant Reprod. 11: 295-296.
Suzuki, G., Watanabe, M., Kai, N., Matsuda, N., Toriyama, K., Takayama, S., Isogai, A. and Hinata, K. (1997) Three members of the S-multigene family are linked to the S locus of Brassica. Mol. Gen. Genet. 256: 257-264.
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Suzuki, G., Watanabe, M., Toriyama, K., Isogai, A. and Hinata, K. (1995) Molecular cloning of members of the S-multigene family in self-incompatible Brassica campestris L. Plant Cell Physiol. 36(7):1273-1280.